Staphylococcal Enterotoxin B: Solid-Phase Radioimmunoassay
نویسندگان
چکیده
منابع مشابه
Rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A.
A rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A is described. The assay procedure requires 3 to 4 h for completion by using a competitive inhibition system in which the antibody is attached to bromacetyl cellulose particles. It is accurate to a level of 0.01 mug of enterotoxin A/ml in a variety of media such as ham, milk products, crab meat, custard, etc. No significant in...
متن کاملStaphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food.
An immunoassay employing (125)I labeled enterotoxins A and B and polystyrene tubes coated with specific antibodies was used for detection and quantitation of enterotoxin in food. Ham salad, cheddar cheese, custard, condensed milk, and salami were studied. Enterotoxin was successfully determined in all the foods by simple extraction procedures. The assay was sensitive to 1 to 10 ng of toxin per ...
متن کاملNeutralization of Staphylococcal enterotoxin B by aptamer
12 Staphylococcal enterotoxin B (SEB) is a major virulence factor for 13 Staphylococcal toxic shock syndrome (TSS). SEB activates a large subset of the T 14 lymphocytic population, releasing pro-inflammatory cytokines. Blocking 15 SEB-initiated toxicity may be an effective strategy for treating TSS. Using a 16 systematic evolution of ligands by exponential enrichment (SELEX) process, we 17 iden...
متن کاملPurification of Type B Staphylococcal Enterotoxin.
Frea, James I. (University of Wisconsin, Madison), Elizabeth McCoy, and F. M. Strong. Purification of type B staphylococcal enterotoxin. J. Bacteriol. 86:1308-1313. 1963.-A procedure for the production and isolation of type B staphylococcal enterotoxin in yields up to 4 mg per liter of toxic culture is presented. Staphylococcus aureus S6 was grown in shake flask cultures at 37 C for 24 hr in a ...
متن کاملA DNA Spiegelmer to staphylococcal enterotoxin B.
Bacterial staphylococcal enterotoxin B is involved in several severe disease patterns and it was therefore used as a target for the generation of biologically stable mirror-image oligonucleotide ligands, so called Spiegelmers. The toxin is a 28 kDa protein consisting of 239 amino acids. Since the full-length protein is not accessible to chemical peptide synthesis, a stable domain of 25 amino ac...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1971
ISSN: 0003-6919
DOI: 10.1128/aem.22.5.837-841.1971